Supplementary Table 2.1
Overview of samples collected for Illumina short read mRNA sequencing and used to create pooled, normalised mRNA and total RNA cDNA libraries for
454 EST sequencing. %Q20 plus indicates the percentage of reads with a quality score >20.
Sample
Tissue
Sample description
Sample date
Total Reads
Total bp
% Q20+
GC %
Z4006TR01
male cones
Immature male cones
May 27, 2010
24,827,234
4,965,446,800
97.81%
45.91%
Z4006TR02
shoots
Vegetative shoots produced in 2010
May 27, 2010
23,797,169
4,759,433,800
97.80%
45.92%
Z4006TR03
needles
Needles produced in 2009
May 27, 2010
24,188,191
4,837,638,200
97.55%
46.49%
Z4006TR04
needles
Needles produced in 2008
May 27, 2010
23,095,399
4,619,079,800
97.47%
46.31%
Z4006TR05
needles
Infected needles produced in 2009
May 25, 2010
23,504,948
4,700,989,600
97.94%
46.60%
Z4006TR07
shoots
Vegetative shoots produced in 2010
June 21, 2010
24,025,688
4,805,137,600
98.05%
46.00%
Z4006TR08
pineapple galls
Pineapple galls
June 21, 2010
23,345,334
4,669,066,800
97.54%
45.29%
Z4006TR09
buds
Buds, early season developing
August 12, 2010
24,594,322
4,918,864,400
97.55%
46.34%
Z3001TR10
female cone
Immature female cone
June 9, 2010
24,142,621
4,828,524,200
97.51%
45.90%
Z4006TR11
needles
Needles from vegetative shoots produced in 2010
August 12, 2010
24,537,590
4,907,518,000
95.48%
44.39%
Z4006TR12
stem
Stem from vegetative shoots produced in 2010
August 12, 2010
23,287,473
4,657,494,600
97.15%
45.34%
Z4006TR13
needles
Needles from vegetative shoots produced in 2010
September 7, 2010
24,077,054
4,815,410,800
94.81%
45.11%
Z4006TR15
buds
Buds, late season developed
September 7, 2010
23,846,759
4,769,351,800
97.13%
45.69%
Z4006TR16
needles
Needles from dried twig (2 days on bench)
September 9, 2010
23,178,302
4,635,660,400
97.09%
44.86%
Z4006TR18
needles
Needles from girdled twig (sampled 1 week after girdling)
September 13, 2010
24,061,272
4,812,254,400
98%
45.00%
Z4006TR19
stem
Stem from girdled twig (sampled 1 week after girdling)
September 13, 2010
21,961,993
4,392,398,600
94.66%
45.33%
Z4006TR20
needles
Early morning (05:30)(dawn) needles from 2010
September 13, 2010
23,174,557
4,634,911,400
93.78%
44.38%
Z4006TR21
needles
Midzday (12:00) needles from 2010
September 13, 2010
23,465,880
4,693,176,000
97.08%
45.93%
Z4006TR22
needles
Late afternoon (19:30)(dusk) needles from 2010
September 13, 2010
23,309,640
4,661,928,000
97.29%
45.16%
Z4006TR23
needles
Night (23:30) needles from 2010
September 13, 2010
23,795,641
4,759,128,200
97.35%
45.15%
Z4006TR24
wood
Wood (phloem+cambium+xylem, early)
June 21, 2010
23,352,912
4,670,582,400
97.36%
45.41%
Z4006TR25
wood
Wood (phloem+cambium+xylem, late)
August 12, 2010
24,830,162
4,966,032,400
97.82%
44.60%
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